陈春来

男， 清华大学， 副教授/副研究员/高级工程师或同等级别

学习/工作经历

1999-2003 北京大学化学与分子工程学院 学士
2003-2008 北京大学化学与分子工程学院 博士
2008-2015 University of Pennsylvania 博士后
2015-2018 清华大学生命科学学院 助理教授
2018-至今 清华大学生命科学学院 副教授

研究领域和兴趣

单分子荧光

主要业绩

本人一直聚焦于单分子荧光技术的发展和应用，立足于对物理化学和化学生物学的深入理解，从探针优化、特异性共价交联到荧光精准测量等多个层面开发独特的新型技术，并在此基础上重点关注结构生物学技术手段无法解析的分子机器的重要亚稳态和动态过程，阐释相应的分子机制和自然规律。主要成果包括：高光稳定性标记策略、新型蛋白质和RNA标记技术、高浓度下的单分子光激活FRET技术、高时间空间分辨率的扫描FRET-FCS技术和精准测量纳米尺度下生物大分子凝集体的dcFCCS技术等；利用单分子荧光揭示了Cas9和Cas12a蛋白的靶标搜索、识别和切割校验机制，G蛋白偶联受体的自抑制和功能选择性的动态调控机制等，并在新机制的指导下实现了Cas蛋白功能优化。相关工作发表在Nature、PNAS、JACS、Angew Chem、Chem Sci、Nat Commun等高水平学术期刊。

代表成果

1. Yu Miao (#), Yuxiao Cheng (#), Yushi Xia, Yongzhen Hei, Wenjuan Wang, Qionghai Dai (*), Jinli Suo (*), Chunlai Chen (*), (2025) Supervised multi-frame dual-channel denoising enables long-term single-molecule FRET under extremely low photon budget, Nat Commun, 16(1), 74

2. Sudheer K. Cheppali (#), Chang Li (#), Wenjing Xing (#), Ruirui Sun, Mengyi Yang, Yi Xue, Si-Yao Lu, Jun Yao, Shan Sun (*), Chunlai Chen (*), Sen-Fang Sui (*), (2024) Single-molecule two- and three-colour FRET studies reveal a transition state in SNARE disassembly by NSF, Nat Commun, 16(1), 250

3. Jiawei Zhao (#), Matthias Elgeti (#)(*), Evan S. O’Brien, Cecília P. Sár, Amal EI Daibani, Jie Heng, Xiaoou Sun, Elizabeth White, Tao Che, Wayne L. Hubbell, Brian K. Kobilka (*), and Chunlai Chen (*), (2024) Ligand efficacy modulates conformational dynamics of the µ-opioid receptor, Nature, 629, 474–480

4. Shouyue Zhang (#), Ao Sun (#), Jing-Mei Qian (#), Shuo Lin (#), Wenjing Xing, Yun Yang, Han-Zhou Zhu, Xin-Yi Zhou, Yan-Shuo Guo, Yun Liu, Yu Meng, Shu-Lin Jin, Cheng-Ping Li, Zhaofu Li, Shuai Jin, Jian-Hua Wang, Meng-Qiu Dong, Caixia Gao, Chunlai Chen (*), Yang Bai (*), Jun-Jie Gogo Liu (*), (2024) Pro-CRISPR PcrIIC1 associated Cas9 system for enhanced bacterial immunity, Nature, 630, 484-492

5. Wenjing Xing (#), Danyuan Li (#), Wenjuan Wang, Jun-Jie Gogo Liu (*), Chunlai Chen (*), (2024) Conformational dynamics of CasX (Cas12e) in mediating DNA cleavage revealed by single-molecule FRET, Nucleic Acids Research, 52, 9014-9027

6. Shuqi Zhou, Yu Miao, Haoren Qiu, Yuan Yao, Wenjuan Wang, Chunlai Chen (*), (2024) Deep learning based local feature classification to automatically identify single molecule fluorescence events, Communications Biology, 7, 1404

7. Danyuan Li (#), Shouyue Zhang (#), Shuo Lin (#), Wenjing Xing (#), Yun Yang, Fengxia Zhu, Dingding Su (*), Chunlai Chen (*), Jun-Jie Gogo Liu (*), (2024) Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavage, Nat Commun, 15, 10727

8. Yuan Zhang (#), Chen Yang (#), Sijia Peng, Jing Ling, Peng Chen, Yumiao Ma, Wenjuan Wang, Zhixing Chen (*), and Chunlai Chen (*), (2023) General strategy to improve the photon budget of thiol-conjugated cyanine dyes, Journal of the American Chemical Society, 145, 7, 4187-4198

9. Ruirui Sun, Yuqian Zhao, Wenjuan Wang, Jun-Jie Gogo Liu, and Chunlai Chen (*), (2023) Nonspecific interactions between Cas12a and dsDNA located downstream of the PAM mediate target search and assist AsCas12a for DNA cleavage, Chem Sci, 14, 3839-3851

10. Xiaolin Niu, Zhonghe Xu, Yufan Zhang, Xiaobing Zuo, Chunlai Chen (*) and Xianyang Fang (*), (2023) Structural and dynamic mechanisms for coupled folding and tRNA recognition of a translational T-box riboswitch, Nat Commun, 14(1), 7394

*以上信息由高级会员个人更新和维护。