凌连生

男， 中山大学化学学院， 教授/研究员/教授级高工或同等级别

学习/工作经历

1991.9 - 1995.7： 湖北师范学院化学系，本科学生
1995.9 -1997.7： 武汉大学化学系，硕士研究生
1997.9 - 2000.7： 武汉大学化学系，博士研究生
2000.9 - 2002.12：中国科学院化学研究所，博士后
2003.1-2004.7：德国马普高分子研究所，博士后
2005.2 - 2011.12：中山大学化学学院，副教授
2012.1 - 至今：中山大学化学学院，教授

研究领域和兴趣

光谱分析化学和光学生物传感器

主要业绩

围绕DNA分析、荧光分析、动态光散射、等温核酸放大技术和PCR等技术的生物传感器开展工作。发表SCI论文80篇，SCI引用1600余篇次。申请中国发明专利9件，授权3件。

代表成果

1. Zou, L.*, Li, X. H., Zhang, J.*, Ling, L.S.* A Highly Sensitive Catalytic Hairpin Assembly-Based Dynamic Light-Scattering Biosensors for Telomerase Detection in Bladder Cancer Diagnosis.. Anal. Chem., 2020, 92(18),12656-12662.
2. Wang, J., Li, T. T, Shen, R. D., Li, G. K., Ling, L. S.* Polymerase Chain Reaction-Dynamic Light Scattering sensor for DNA and protein by using both replication and cleavage properties of Taq polymerase. Anal. Chem., 2019, 91,3429-3435.
3. Wang, J., Zhang, J., Li, T.T., Li, G.K., Ling, L.S.* Non-Invasive diagnosis of bladder cancer in human urine based upon strand displacement amplification and dynamic light scattering. Biosensor & Bioelectronics, 2019, 131,143-148.
4. Wang, J., Li, T.T, Li, H.G., Li, G.K., Wu, S.X.*, Ling, L.S.*, A universal colorimetric PCR biosensor based upon triplex formation with the aid of Ru(phen)2dppx2+. Sensors & Actuators: B. Chemical. 2019, 278, 39-45.
5. Zou, L., Li, X., Li, T.T., Ling, L.S.* Hybridization chain reaction and DNAzyme-based dual signal amplification strategy for sensitive colorimetric sensing of acetylcholinesterase activity and inhibitor screening in rat blood. Sensors & Actuators: B. Chemical. 2018, 267, 272-278.
6. Zou, L., Ling, L.S.* Ultrasensitive detection of HIV DNA with polymerase chain reaction-dynamic light scattering. Anal. Chem., 2018, 90,13373-13377.
7. Li, R.M., Zhou, Y., Zou, L., Li, S.M., Shu, C.Y., Wang, C.R., Ge, J.C.*, Ling,L.S.* In situ growth of gold nanoparticles on hydrogen-bond supramolecular structures with high peroxidase-like activity at neutral pH and their application to one-pot blood glucose sensing. Sensors & Actuators: B. Chemical. 2017, 245, 656-664.
8. Zou, L., Li, R. M., Zhang, M. J., Luo, Y. W., Zhou, N., Wang, J., Ling,L.S.* Colorimetric sensing platform based upon recognizing hybridization chain reaction product with oligonucleotide modified gold nanoparticles through triplex formation. Nanoscale, 2017, 9, 1986 - 1992.
9. Luo, Y. W., Li, R. M., Ling, L.S.* An Ag+-stabilized triplex DNA molecular switch controlled hybridization chain reaction. SCIENCE CHINA Chemistry, 2017, 60,12,1575-1580.
10. Zhang, M.J., Li R.M., Wang, J., Ling, L.S.* Sequence-specific Recognition of HIV-1 DNA Based upon Nicking-Assisted Strand Displacement Amplification and Triple Helix DNA. SCIENCE CHINA Chemistry, 2017, 60(11), 1468-1473.
11. Li, Z.B., Miao, X.M.,*，Xing K.，Xue, P.，Zhu, A.H.*, Ling,L.S.*，Ultrasensitive electrochemical sensor for Hg2+ by using hybridization chain reaction coupled with Ag@Au core-shell nanoparticles. Biosensors and Bioelectronics，2016，80, 339-343.
12. Li Z.B., Miao, X.M.,*，Xing K.，Zhu, A.H., Ling,L.S.*，Enhanced electrochemical recognition of double-stranded DNA by using hybridization chain reaction and positively charged gold nanoparticles. Biosensors and Bioelectronics，2015, 74, 687-690.
13．Miao, X.M.,* Guo, X.T.，Xiao, Z.Y., Ling, L.S.* Electrochemical molecular beacon biosensor for sequence-specific recognition of double-stranded DNA. Biosensors and Bioelectronics，2014，59, 54-57.
14. Miao, X.M.,* Zou, S.Y.，Zhang, H.，Ling, L.S.*，Highly sensitive carcinoembryonic antigen detection using Ag@Au core-shell nanoparticles and dynamic light scattering. Sensors and Actuators B: Chemical，2014, 191, 396-400.
15. Miao, X. M., Ling, L.S.,* Shuai, X.T.* Sensitive detection of glucose in human serum with Oligonucleotide modified gold nanoparticles by using dynamic light scattering technique. Biosensor and Bioelectronics, 2013, 41, 880-883.
16. Xiao, Z. Y., Guo, X. T., Ling,L.S.* Sequence-specific recognition of double-stranded DNA with molecular beacon with the aid of Ag+ under neutral pH environment. Chemical Communications, 2013, 49 (34), 3573 - 3575.
17. Miao, X. M., Ling, L.S.,* Shuai, X.T.* Ultrasensitive detection of lead(II) with DNAzyme and gold nanoparticles probes by using a dynamic light scattering technique. Chemical Communications, 2011, 47 (14), 4192 – 4194.
18. Xin, A., Dong, Q. P., Xiong, C., Ling, L.S.* Colorimetric recognition of DNA intercalators with unmodified gold nanoparticles. Chemical Communications, 2009, 1658-1660.
19. Ling, L.S., Butt, H. J., Berger, R.* The rupture force between the third strand and double strand within a triplex DNA. J. Am. Chem. Soc. 2004, 126, 13992-13997.

20.凌连生，李海刚，王京， 一种多倍PCR扩增方法。 中国发明专利（授权），ZL201710591161.6
21.凌连生，邹李，一种实时动态光散射PCR仪。 中国发明专利（授权），ZL201810282834.4
22.凌连生，邹李，一种基于金纳米颗粒的定量PCR检测方法。中国发明专利(授权），ZL201810378113.3

*以上信息由高级会员个人更新和维护。