何化

男， 中国石油大学（华东）化学化工学院， 副教授/副研究员/高级工程师或同等级别

学习/工作经历

1998/09-2002/07湖南师范大学应用化学专业，获理学学士学位
2002/09-2005/07湖南师范大学分析化学专业，获理学硕士学位（导师：姚守拙院士）
2005/09-2008/11 上海交通大学应用化学专业，获工学博士学位（导师：任吉存教授）
2008/12-2010/11 中国石油大学（华东）化学化工学院，讲师
2014/02-2015/02 美国斯坦福大学化学系国家公派访问学者（导师：戴宏杰院士）
2010年12月-至今 中国石油大学（华东），化学化工学院，副教授

研究领域和兴趣

单分子荧光技术、纳米生物技术

主要业绩

1) 单分子荧光技术
率先提出基于荧光闪烁的单分子定量超分辨成像新方法，受到国内外同行的关注与认可，至今国内外多个研究小组加入该领域研究和交流； 近5年来，致力于单分子荧光技术与人工智能技术的结合，提高方法的准确性和智能性，并将其用于生物和化学领域研究，目前已取得一系列进展。

2) 功能荧光纳米探针的设计、分子影像及其脑损伤治疗
针对目前脑损伤治疗的难题，设计了一系列基于活性氧氮物种识别的碳纳米酶，可用于颅脑神经损伤部位的精准靶向和高效修复；设计合成了可快速排泄的近红外荧光纳米探针，用于高信噪比活体肿瘤成像。

3) 表面增强荧光成像用于高灵敏生物检测和体外诊断
设计了表面等离激元、光子晶体等纳米镀膜，发展了飞摩尔检测灵敏度的高通量荧光成像技术，用于细胞药物靶点和体外肿瘤标志物（血液中蛋白质、外泌体等）的检测，为疾病的早期预警和治疗提供可行性策略和理论指导。

代表成果

1. 论文：
1) Jian Mao, Minmin Xue, Xin Guan, Qian Wang, Zhirui Wang, Guangyong Qin, and Hua He*. Near-Infrared Blinking Carbon Dots Designed for Quantitative Nanoscopy. Nano Letters, 2023, 23, 1, 124-131. DOI: 10.1021/acs.nanolett.2c03711
2) Hua He*, Guangyong Qin, Simin Bi, Zhenzhen Feng, Jian Mao, Xin Guan, Minmin Xue, zhirui wang, Xiaojuan Wang, Daoyong Yu, and Fang Huang*. Deep-Learning-Enhanced Diffusion Imaging Assay for Resolving Local-Density Effects on Membrane Receptors. Analytical Chemistry, 2023, 95(6), 3300-3308. DOI: 10.1021/acs.analchem.2c04326.
3) Qian Wang, Qian Zhang, Hua He,* Zhenzhen Feng, Jian Mao, Xiang Hu, Xiaoyun Wei, Simin Bi, Guangyong Qin, Xiaojuan Wang, Baosheng Ge, Daoyong Yu, Hao Ren and Fang Huang*. Carbon Dot Blinking Fingerprint Uncovers Native Membrane Receptor Organizations via Deep Learning. Analytical Chemistry 2022, 94(9), 3914-3921. DOI: 10.1021/acs.analchem.1c04947.
4) X. Mu#, J. Wang#, Hua He #, Qifeng Li, Bing Yang, Junhui Wang, Haile Liu, Yalong Gao, Lufei Ouyang, Si Sun, Qinjuan Ren, Xinjian Shi, Wenting Hao, Qiaoman Fei, Jiang Yang, Lulin Li, Ryan Vest, Tony Wyss-Coray, Jian Luo, and Xiaodong Zhang*. An oligomeric semiconducting nanozyme with ultrafast electron transfers alleviates acute brain injury. Science Advances 2021, 7(46), eabk1210. DOI: 10.1126/sciadv.abk1210.
5) Hua He*, Xiaoliang Chen, Zhenzhen Feng, Lihua Liu, Qian Wang, and Simin Bi. Nanoscopic Imaging of Nucleolar Stress Enabled by Protein-Mimicking Carbon Dots. Nano Letters 2021, 21(13), 5689–5696. DOI: 10.1021/acs.nanolett.1c01420.
6) Naseer Ullah Khan, Muhammad Zahir, Xukun Liu, Jing Lin, Qihong Zheng, Huajie Zhang, Samiullah Malik, Hua He *, and Liming Shen*. Ultra-sensitive detection of exosome using biofunctionalized gold nanorods on silver-island film. Nano Letters 2021, 21(13), 5532–5539. DOI: 10.1021/acs.nanolett.1c00830.
7) Qian wang, Hua He *, Qian Zhang, Zhenzhen Feng, Jiqiang Li, Xiaoliang Chen, Lihua Liu, Xiaojuan Wang, Baosheng Ge, Daoyong Yu, Hao Ren and Fang Huang*. Deep-Learning-Assisted Single-Molecule Tracking on a Live Cell Membrane. Analytical Chemistry 2021, 93(25), 8810–8816. DOI: 10.1021/acs.analchem.1c00547.
8) Hua He, Lihua Liu, Xiaoliang Chen, Qian wang, Xiaojuan Wang, Werner M. Nau*, and Fang Huang*. Carbon Dot Blinking Enables Accurate Molecular Counting at Nanoscale Resolution. Analytical Chemistry 2021, 93(8), 3968–3975. DOI: 10.1021/acs.analchem.0c04885.
9) Hua He*, Di Liu, Zhenzhen Feng, Aijun Guo, Lihua Liu, and Xiaoliang Chen. Antifade Carbon Dots on Plasmonic Substrate for Enhanced Protein Detection in Immunotherapy. ACS Sensors 2020, 5(12), 4027–4034. DOI: 10.1021/acssensors.0c01983.
10) Xiaoyu Mu#, Hua He#, Junying Wang#, Wei Long, Qifeng Li, Haile Liu, Yalong Gao, Lufei Ouyang, Qinjuan Ren, Si Sun, Jingya Wang, Jiang Yang, Qiang Liu, Yuanming Sun, Changlong Liu, Xiaodong Zhang*, Wenping Hu. Carbogenic Nanozyme with Ultrahigh RNS Selectivity for Traumatic Brain Injury. Nano Letters 2019, 19(7), 4527-4534. DOI: 10.1021/acs.nanolett.9b01333.
11) Hua He, Xinjian Shi, Junying Wang, Xiaojuan Wang, Qian Wang, Daoyong Yu, Baosheng Ge, Xiaodong Zhang* and Fang Huang*. Reactive Oxygen Species-Induced Aggregation of Nanozymes for Neuron Injury. ACS Applied Materials & Interfaces 2020, 12(1), 209-216. DOI: 10.1021/acsami.9b17509.
12) Hua He, Xu Liu, Shan Li, Xiaojuan Wang, Qian Wang, Jiqiang Li, Junying Wang, Hao Ren, Baosheng Ge, Shengjie Wang, Xiaodong Zhang, Fang Huang, High-Density Super-Resolution Localization Imaging with Blinking Carbon Dots. Analytical Chemistry 2017, 89 (21), 11831-11838. DOI: 10.1021/acs.analchem.7b03567
13) Naseer Ullah Khan, Zhenzhen Feng, Hua He*, Qian Wang, Xu Liu, Shan Li, Xinjian Shi, Xiaojuan Wang, Baosheng Ge and Fang Huang*, A Facile Plasmonic Silver Needle for Fluorescence-Enhanced Detection of Tumor Markers. Analytica Chimica Acta 2018, 1040, 120-127. DOI: 10.1016/j.aca.2018.07.071.
14) Qian wang, Zhenzhen Feng, Hua He*, Jiqiang Li, Xiaoliang Chen, Lihua Liu, Xiaojuan Wang, Baosheng Ge, Daoyong Yu, Hao Ren and Fang Huang*. Nonblinking Carbon Dots for Imaging and Tracking Receptors on Live Cell membrane. Chemical Communications 2021, 57(45), 5554-5557. DOI: 10.1039/d1cc01120k.
15) Naseer Ullah Khan, Hua He*, Xiaojuan Wang, Baosheng Ge, Qian Wang, Xu Liu, Jun Lao, Yanan Wang, Jiqiang Li, Zhencai Wang, Shitan Zhou, and Fang Huang*. A Two-Color Fluorescence Enhanced Dot-Blot Assay for Revealing Co-operative Expression of Chemokine Receptors in Cells. Chemical Communications 2018, 54, 778-781. DOI: 10.1039/c7cc08167g.
16) Hua He, Shan Li, Xinjian Shi, Xiaojuan Wang, Xu Liu, Qian Wang, Aijun Guo, Baosheng Ge, Naseer Ullah Khan, and Fang Huang, Quantitative Nanoscopy of Small Blinking Graphene Nanocarriers in Drug Delivery. Bioconjugate Chemistry 2018, 29 (11), 3658–3666.DOI: 10.1021/acs.bioconjchem.8b00589.
17) Lufei Ouyang, Xiaoyu Mu, Junying Wang, Qifeng Li, Yalong Gao, Haile Liu, Si Sun, Qinjuan Ren, Ruijuan Yan, Jingya Wang, Qiang Liu, Yuanming Sun, Changlong Liu, Hua He*, Wei Long and Xiaodong Zhang*. Carbon dot targeting to nitrogen signaling molecules for inhibiting neuronal death. Journal of Materials Chemistry B 2020, 8(11), 2321-2330. DOI: 10.1039/c9tb02447f.
18) Jun Lao#, Hua He#, Xiaojuan Wang, Zhencai Wang, Yanzhuo Song, Bin Yang, Naseer Ullahkhan, Baosheng Ge*, Fang Huang*. Single-Molecule Imaging Demonstrates Ligand Regulation of Oligomeric Status of CXCR4 in Living Cells. ‎ Journal of Physical Chemistry B 2017, 121 (7), 1466-1474. DOI: 10.1021/acs.jpcb.6b10969.
19) Lingfang Liu, Xiaoyu Mu, Haixia Liu, Qian Wang, Xueting Bai, Junying Wang, Haile Liu, Fujuan Xu, Yaqi Jing, Haitao Dai, Changlong Liu*, Hua He*, and Xiaodong Zhang *, Structure, luminescence, and bioimaging of bimetallic CuAu nanoclusters. Optical Materials 2018, 86, 291–297. DOI: 10.1016/j.optmat.2018.10.025
20) Yuan He, Zhenzhen Feng, Xinjian Shi, Shan Li, Yunguo, Liu*, Guangming Zeng, and Hua He* , N- and O-doped Carbon Dots for Rapid and High-Throughput Dual Detection of Trace Amounts of Iron in Water and Organic Phases. Journal of Fluorescence 2019, 29(1):137-144. DOI: 10.1007/s10895-018-2321-5.
21) Hua He, Zhencai Wang, Tiantian Cheng, Xu Liu, Xiaojuan Wang, Junying Wang, Hao Ren, Yawei Sun, Yanzhuo Song, Jiang Yang, Yongqing Xia, Shengjie Wang, Xiaodong Zhang*, and Fang Huang*. Visible and Near-Infrared Dual-Emission Carbogenic Small Molecular Complex with High RNA Selectivity and Renal Clearance for Nucleolus and Tumor Imaging. ACS Applied Materials & Interfaces 2016, 8 (42), 28529-28537. DOI: 10.1021/acsami.6b10737.
22) Hua He, Min Feng, Jing Hu, Cuixia Chen, Jiqian Wang, Xiaojuan Wang, Hai Xu*, Jian R. Lu*. Designed Short RGD Peptides for One-Pot Aqueous Synthesis of Integrin-binding CdTe and CdZnTe Quantum Dots. ACS Applied Materials & Interfaces 2012, 4, 6362-6370. DOI: 10.1021/am3020108.
23) Hua He, Huifeng Qian, Chaoqing Dong, Kanglin Wang, Jicun Ren*. Single nonblinking CdTe quantum dots synthesized in aqueous thiopropionic acid. Angewandte Chemie International Edition 2006, 45(45): 7588-7591. DOI: 10.1002/anie.200602758.
24) Hua He, Chao Xie, Jicun Ren*. Non-Bleaching Fluorescence of Gold Nanoparticles and Its Applications in Cancer Cell Imaging. Analytical Chemistry 2008, 80(15): 5951-5957.DOI: 10.1021/ac8005796.
25) Hua He, Jicun Ren*. A Novel Evanescent Wave Scattering Imaging Method for Single Gold Particle Tracking in Solution and on Cell Membrane. Talanta 2008, 77(1):166-171. DOI: 10.1016/j.talanta.2008.05.059.
26) Hua He, Xiaojuan Wang, Tiantian Cheng, Yongqing Xia, Jun Lao, Baosheng Ge, Hao Ren, Naseer Ullah Khan, Fang Huang*. An Ultra-High Fluorescence Enhancement and High Throughput Assay for Revealing Expression and Internalization of Chemokine Receptor CXCR4. Chemistry-A European Journal 2016, 22, 5863-5867. DOI: 10.1002/chem.201600245
27) Hua He *, Xiaojuan Wang, Zhenzhen Feng, Tiantian Cheng, Xing Sun, Yawei Sun, Yongqing Xia, Shengjie Wang, Junying Wang, Xiaodong Zhang*. Rapid microwave-assisted synthesis of ultra-bright fluorescent carbon dots for live cell staining, cell-specific targeting and in vivo imaging. Journal of Materials Chemistry B 2015, 3, 4786-4789. DOI: 10.1039/c5tb00570a.
28) Hua He, Qingji Xie, Shouzhuo Yao*. An electrochemical quartz crystal impedance study on anti-human immunoglobulin G immobilization in the polymer grown during dopamine oxidation at an Au electrode. Journal of Colloid and Interface Science 2005, 289, 446-454. DOI: 10.1016/j.jcis.2005.03.085.
29) Hua He, Xing Sun, Xiaojuan Wang, Yawei, Sun, Hai Xu*, Jian R. Lu*. Cyclic Arginyl-Glycyl-Aspartic Acid (RGD) Peptide-Induced Synthesis of Uniform and Stable One-Dimensional CdTe Nanostructures in Aqueous Solution. RSC Advances 2014, 4, 11794-11797. DOI: 10.1039/C3RA47253A
30) Hua He*, Xing Sun, Xiaojuan Wang and Hai Xu*, Synthesis of Highly Luminescent and Biocompatible CdTe/CdS/ZnS Quantum Dots Using Microwave Irradiation: a Comparative Study of Different Ligands, Luminescence 2014, 29, 837–845. DOI: 10.1002/bio.2630.

2. 专利
1）一种双效荧光增强的免疫分析方法 , 专利号： ZL 202010520800.1, 第一发明人 何化
2）一种受自由基诱导聚集的治疗性纳米酶及其制备方法 ，专利号 ZL201910660001.1 第一发明人 何化

3. 论著：
1) Hua He, Qian Wang, Xiaoqiang Wang, Fang Huang. Single molecule fluorescence for studying the membrane protein dynamics and interactions, in: Biomembranomics: Structure, Techniques, and Applications, ‎ Jenny Stanford Publishing 2023, Chapter 6, 201-237. 受邀撰写
2) Hua He, Xiaojuan Wang, Fang Huang. Analysis and Applications of Single Molecule Fluorescence in Live Cell Membranes, in: ‎Membrane Biophysics: New Insights and Methods, ‎Springer 2018, Chapter 6, 147-173. 受邀撰写

*以上信息由高级会员个人更新和维护。