张锴

男， 天津医科大学， 教授/研究员/教授级高工或同等级别

学习/工作经历

2014-01 至今 天津医科大学，基础医学院，教授
2011-06 至 2011-08 美国芝加哥大学，Benmay癌症研究所，访问学者
2008-04 至 2013-12 南开大学，化学学院， 副教授
2006-09至 2008-03 美国得州大学西南医学中心（达拉斯）
2004-12至 2006-09 美国华盛顿州立大学
2003-11至 2004-12 法国国家科研中心 CNRS-7575（巴黎）
2000-09 至 2003-07 南开大学， 有机化学，博士
1997-09 至 2000-07 南开大学， 有机化学，硕士
1992-09 至 1996-07 南开大学， 化学， 学士

研究领域和兴趣

蛋白质组学、质谱、色谱、化学生物学

主要业绩

长期从事“生物色谱质谱和蛋白质组学”研究，聚焦于“蛋白质赖氨酸修饰的组学鉴定与应用”，累计发表相关SCI论文140多篇，主要成果以通讯作者发表在Nature Chemical Biology、Nature Communications、Nature Micrology、Science Advances、Molecular Cell、PNAS、JACS、Analytical Chemistry等国际主流学术期刊。近年来的主要成果包括：
（1）发展了组蛋白修饰和调控元件的系列分析新策略、新方法。率先建立了“基于多价态亲和光交联的组蛋白修饰调控蛋白分析新方法”，显著提升了阅读器蛋白的鉴定效率与准确性（Anal Chem 2018; Sci Adv 2019; PNAS 2024）；发展了“基于遗传密码扩展非天然氨基酸的邻近标记新技术”，率先实现细胞内组蛋白阅读器蛋白的捕获与鉴定；提出了“基于邻近标记的正交捕获亲和富集”（ProLORT）新策略，实现了活细胞内酶调控蛋白质修饰位点的精准鉴定（Cell Chem Biol 2024）。
（2）发现并系统揭示了乳酰化、β-羟基丁酰化、琥珀酰化等新型蛋白质赖氨酸修饰的组学特征、新的调控元件和分子机制。率先发现了乳酰转移酶YiaC和HBO1（Nat Commun 2022、2024）、阅读器DPF2（PNAS 2024）；率先发现了β-羟基丁酰化供体的关键辅酶合成酶ACSS2、修饰转移酶KAT7（Sci Adv 2025），阅读器ENL（Nucleic Acids Res 2024）；率先发现了原核细胞中的2-羟基异丁酰转移酶TmcA（Nat Chem Biol 2022）、琥珀酰转移酶CitC（Nat Microbiol 2025）等。
（3）系统研究了食管癌等肿瘤细胞蛋白质赖氨酸修饰的组学特征，揭示了异常蛋白质修饰的肿瘤生物学功能。首次报道组蛋白乳酰化阅读器DPF2促进肿瘤进展的新机制（PNAS 2024）；率先揭示HBO1催化组蛋白H3K9la促进癌基因表达（Nat Commun 2024）； 发现了ACSS2协同KAT7调控组蛋白β-羟基丁酰化促进肿瘤表观重编程（Sci Adv 2025）。

代表成果

1. Chen, C.*; Zhang, X.; Bai, X.; Zang, Y.; Fan, Z.; Zhang, J.; Wang, J. N.; Han, Y.; Zhang, K.*, Discovery and Enzymatic Regulation of Lysine Fumarylation, a Post-Translational Modification in Bacteria. Journal of the American Chemical Society 2026, 148 (15), 16502. DOI10.1021/jacs.6c04986.
2. Wang, S.; Niu Z.; Zhang, Y.; Liu R.; Zhu, R.; Sun yuxuan; Han Y.; Wang, A’; Zhang, J.; Zhang, H.; Zang, Y.; Zhao, Y.; Zhai, G.; Zhang, K.*, ACSS2 coupled with KAT7 regulates histone beta-hydroxybutyrylation to enhance transcription. Science Advances 2025, 11(33): eadv8448. DOI10.1126/sciadv.adv8448.
3. Li, L.; Liu, Y.; Liu, D.; Wang, J.; Wang, M.; Xiang, B.; Qin, J.; Yao, T.; Li, W.; Wu, P.; Wang, Q.; Zhang, J.; Xu, Y.; Liu, M.; Wang, Y.; Ma, G.; Liu, R.; Li, X.; Huai, Z.; Huang, Y.; Guo, H.; Yang, B.; Feng, L.; Huang, D.*; Zhang, K.*; Wang, L.*; Liu, B.*, Microbiota-derived succinate promotes enterohaemorrhagic Escherichia coli virulence via lysine succinylation. Nature Microbiology 2025, (3):749-764. DOI10.1038/s41564-025-01931-x.
4. Cao, Y. F.; Wang, H.; Sun, Y.; Tong, B. B.; Shi, W. Q.; Peng, L.; Zhang, Y. M.; Wu, Y. Q.; Fu, T.; Zou, H. Y.; Zhang, K.*; Xu, L. Y.*; Li, E. M.*, Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression. Nat Commun 2025, 16 (1) 1271. DOI10.1038/s41467-025-56645-9.
5. Zhai, G.; Niu, Z.; Jiang, Z.; Zhao, F.; Wang, S.; Chen, C.; Zheng, W.; Wang, A.; Zang, Y.; Han, Y.; Zhang, K.*, DPF2 reads histone lactylation to drive transcription and tumorigenesis. PNAS 2024, 121 (50), e2421496121. DOI10.1073/pnas.2421496121.
6. Niu, Z.; Chen, C.*; Wang, S.; Lu, C.; Wu, Z.; Wang, A.; Mo, J.; Zhang, J.; Han, Y.; Yuan, Y.; Zhang, Y.; Zang, Y.; He, C.; Bai, X.; Tian, S.; Zhai, G.; Wu, X.; Zhang, K.*, HBO1 catalyzes lysine lactylation and mediates histone H3K9la to regulate gene transcription. Nat Commun 2024, 15 (1), 3561. DOI10.1038/s41467-024-47900-6.
7. Huang, Y.; Zhai, G.*; Fu, Y.; Li, Y.; Zang, Y.; Lin, Y.*; Zhang, K.*, A proximity labeling-based orthogonal trap strategy identifies HDAC8 promotes cell motility by modulating cortactin acetylation. Cell Chemical Biology 2024, 31 (3), 514-522 e4. DOI10.1016/j.chembiol.2024.02.003.
8. Hanyang Dong; Yujie Zhao; Changfen Bi; Yue Han; Jianji Zhang; Xue Bai; Guijin Zhai; Hui Zhang; Shanshan Tian; Deqing Hu; Liyan Xu; Kai Zhang*, TmcA functions as a lysine 2-hydroxyisobutyryltransferase to regulate transcription, Nature Chemical Biology, 2022, 18(2), 142-151. DOI10.1038/s41589-021-00906-3.
9. Hanyang Dong; Jianji Zhang; Hui Zhang; Yue Han; Congcong Lu; Chen Chen; Xiaoxia Tan; Siyu Wang; Xue Bai; Guijin Zhai; Shanshan Tian; Tao Zhang; Zhongyi Cheng; Enmin Li; Liyan Xu*; Kai Zhang*, YiaC and CobB regulate lysine lactylation in Escherichia coli, Nature Communications, 2022, 13, 6628. DOI10.1038/s41467-022-34399-y.
10. Zhao, J., Tian, S., Guo, Q., Bao, K., Yu, G., Wang, X., Shen, X., Zhang, J., Chen, J., Yang, Y., Liu, L., Li, X., Hao, J., Yang, N., Liu, Z., Ai, D., Yang, J., Zhu, Y., Yao, Z., Ma, S.*, Zhang, K.*, and Shi, L.* (2022) A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination. Molecular Cell, 82, 2571-2587. DOI10.1016/j.molcel.2022.04.031.
11. Yepei Huang; Guijin Zhai*; Yanan Li; Yue Han; Chen Chen; Congcong Lu; Kai Zhang*, Deciphering the Interactome of Histone Marks in Living Cells via Genetic Code Expansion Combined with Proximity Labeling, Analytical Chemistry, 2022, 94, 10705-10714. DOI10.1021/acs.analchem.2c01042.
12. Tian, S. S., Liu, R. R., Wu, Z. Y., and Zhang, K.* (2022) A Novel Strategy Based on Permanent Protein Modifications Induced by Formaldehyde for Food Safety Analysis. Analytical Chemistry, 94, 17365–17369. DOI10.1021/acs.analchem.2c04069.
13. Chen, P., Zhuo, Y., Tian, S. S., Zhang, T., Zhai, G. J., Fan, E. G., Ma, Z. Y., Zhang, Y. K., and Zhang, K.* (2020) An Integrated Approach for Combinatorial Readout of Dual Histone Modifications by Epigenetic Tandem Domains. Analytical Chemistry, 92, 6218-6223. DOI10.1021/acs.analchem.9b05394.
14. Zhenchang Guo; Feng Pan; Liu Peng; Shanshan Tian; Jiwei Jiao; Liandi Liao; Congcong Lu; Guijin Zhai; Zhiyong Wu; Hanyang Dong; Xiue Xu; Jianyi Wu; Pu Chen; Xue Bai; Dechen Lin; Liyan Xu*; Emin Li*; Kai Zhang*, Systematic Proteome and Lysine Succinylome Analysis Reveals Enhanced Cell Migration by Hyposuccinylation in Esophageal Squamous Cell Carcinoma. Molecular & Cellular Proteomics, 2021, 20, 100053. DOI10.1074/mcp.RA120.002150.
15. Hanyang Dong; Guijin Zhai; Cong Chen; Xue Bai; Shanshan Tian; Deqing Hu; Enguo Fan; Kai Zhang*, Protein lysine de-2-hydroxyisobutyrylation by CobB in prokaryote. Science Advances 2019, 5: eaaw6703. DOI10.1126/sciadv.aaw6703.

*以上信息由高级会员个人更新和维护。